학술논문

Loop-Mediated Isothermal Amplification for Salmonella Detection in Food and Feed: Current Applications and Future Directions.
Document Type
Article
Source
Journal of Experimental Biology. Jun2018, Vol. 221 Issue 11, p309-331. 23p.
Subject
*SALMONELLA detection
*FOOD microbiology
*FOOD safety
*ANIMAL feeds
*PUBLIC health
*SAFETY
Language
ISSN
0022-0949
Abstract
Loop-mediated isothermal amplification (LAMP) has become a powerful alternative to polymerase chain reaction (PCR) for pathogen detection in clinical specimens and food matrices. Nontyphoidal Salmonella is a zoonotic pathogen of significant food and feed safety concern worldwide. The first study employing LAMP for the rapid detection of Salmonella was reported in 2005, 5 years after the invention of the LAMP technology in Japan. This review provides an overview of international efforts in the past decade on the development and application of Salmonella LAMP assays in a wide array of food and feed matrices. Recent progress in assay design, platform development, commercial application, and method validation is reviewed. Future perspectives toward more practical and wider applications of Salmonella LAMP assays in food and feed testing are discussed. [ABSTRACT FROM AUTHOR]