학술논문
Exercise dose affects the circulating microRNA profile in response to acute endurance exercise in male amateur runners.
Document Type
Article
Author
Fernández‐Sanjurjo, Manuel; Úbeda, Natalia; Fernández‐García, Benjamín; del Valle, Miguel; Ramírez de Molina, Ana; Crespo, María Carmen; Martín‐Hernández, Roberto; Casas‐Agustench, Patricia; Martínez‐Camblor, Pablo; de Gonzalo‐Calvo, David; Díez‐Robles, Sergio; García‐González, Ángela; Montero, Ana; González‐González, Felipe; Rabadán, Manuel; Díaz‐Martínez, Ángel Enrique; Whitham, Martin; Iglesias‐Gutiérrez, Eduardo; Dávalos, Alberto
Source
Subject
*BIOMARKERS
*CELL cycle
*CELL communication
*CELLULAR signal transduction
*ENDURANCE sports training
*ENERGY metabolism
*GENE expression
*POLYMERASE chain reaction
*RUNNING
*AMATEUR athletes
*PRE-tests & post-tests
*LONG-distance running
*REVERSE transcriptase polymerase chain reaction
*EXERCISE intensity
*RNA probes
*MICRORNA
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Language
ISSN
0905-7188
Abstract
The systemic response to exercise is dose‐dependent and involves a complex gene expression regulation and cross‐talk between tissues. This context ARISES the need for analyzing the influence of exercise dose on the profile of circulating microRNAs (c‐miRNAs), as emerging posttranscriptional regulators and intercellular communicators. Thus, we hypothesized that different exercise doses will determine specific c‐miRNA signatures that will highlight its potential as exercise dose biomarker. Nine active middle‐aged males completed a 10‐km race (10K), a half‐marathon (HM), and a marathon (M). Blood samples were collected immediately before and after races. Plasma RNA was extracted, and a global screening of 752 microRNAs was analyzed using RT‐qPCR. Three different c‐miRNA profiles were defined according to the three doses. In 10K, 14 c‐miRNAs were found to be differentially expressed between pre‐ and post‐exercise, 13 upregulated and 1 downregulated. Regarding HM, 13 c‐miRNAs were found to be differentially modulated, in all the cases upregulated. A total of 28 c‐miRNAs were found to be differentially expressed in M, 21 overexpressed and 7 repressed after this race. We had also found 3 common c‐miRNAs between 10K and M and 2 common c‐miRNAs between 10K and HM. In silico analysis supported a close association between exercise dose c‐miRNA profiles and cellular pathways linked to energy metabolism and cell cycle. In conclusion, we have observed that different exercise doses induced specific c‐miRNA profiles. So, our results point to c‐miRNAs as emerging exercise dose biomarkers and as one of regulatory mechanisms modulating the response to endurance exercise. [ABSTRACT FROM AUTHOR]