학술논문
Artificial intelligence based modeling and optimization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production process by using Azohydromonas lata MTCC 2311 from cane molasses supplemented with volatile fatty acids: A genetic algorithm paradigm
Document Type
Article
Source
Subject
*POLY-beta-hydroxybutyrate
*MOLASSES
*FATTY acids
*GENETIC algorithms
*MATHEMATICAL optimization
*ARTIFICIAL neural networks
*RESPONSE surfaces (Statistics)
*PROPIONIC acid
*PREDICTION models
*
*
*
*
*
*
*
*
Language
ISSN
0960-8524
Abstract
Abstract: The present work describes the optimization of medium variables for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] by Azohydromonas lata MTCC 2311 using cane molasses supplemented with propionic acid. Genetic algorithm (GA) has been used for the optimization of P(3HB-co-3HV) production through the simulation of artificial neural network (ANN) and response surface methodology (RSM). The predictions by ANN are better than those of RSM and in good agreement with experimental findings. The highest P(3HB-co-3HV) concentration and 3HV content have been reported as 7.35g/l and 16.84mol%, respectively by hybrid ANN–GA. Upon validation, 7.20g/l and 16.30mol% of P(3HB-co-3HV) concentration and 3HV content have been found in the shake flask, whereas 6.70g/l and 16.35mol%, have been observed in a 3l bioreactor, respectively. The specific growth rate and P(3HB-co-3HV) accumulation rate of 0.29 per h and 0.16g/lh determined with cane molasses are comparable to those observed on pure substrates. [Copyright &y& Elsevier]