학술논문
PROCESS PARAMETERS STUDY OF α-AMYLASE PRODUCTION IN A PACKED-BED BIOREACTOR UNDER SOLID-STATE FERMENTATION WITH POSSIBILITY OF TEMPERATURE MONITORING.
Document Type
Article
Source
Subject
*AMYLASES
*BIOREACTORS
*CHEMICAL reactors
*BACILLUS (Bacteria)
*SOLID-state fermentation
*FERMENTATION
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Language
ISSN
1082-6068
Abstract
Production of α-amylase in a laboratory-scale packed-bed bioreactor by Bacillus sp. KR-8104 under solid-state fermentation (SSF) with possibility of temperature control and monitoring was studied using wheat bran (WB) as a solid substrate. The simultaneous effects of aeration rate, initial substrate moisture, and incubation temperature on α-amylase production were evaluated using response surface methodology (RSM) based on a Box–Behnken design. The optimum conditions for attaining the maximum production of α-amylase were 37°C, 72% (w/w) initial substrate moisture, and 0.15 L/min aeration. The average enzyme activity obtained under the optimized conditions was 473.8 U/g dry fermented substrate. In addition, it was observed that the production of enzyme decreased from the bottom of the bioreactor to the top. [ABSTRACT FROM PUBLISHER]