학술논문
Site-specific labeling of ‘second generation’ annexin V with 99m Tc(CO)3 for improved imaging of apoptosis in vivo
Document Type
Article
Author
Source
Subject
*ANNEXINS
*APOPTOSIS
*BINDING sites
*TARGETED drug delivery
*DRUG labeling
*TECHNETIUM
*MEDICAL imaging systems
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Language
ISSN
0968-0896
Abstract
Abstract: In this study ‘second generation’ AnxV was specifically labeled with 99mTc in three different ways outside the binding region of the protein to obtain an improved target-to-background activity ratio. The compounds were tested in vitro and in vivo in normal mice and in a model of hepatic apoptosis (anti-Fas mAb). The apoptosis binding was most prominent for the HIS-tagged ‘second generation’ AnxV labeled with 99mTc(CO)3 in comparison to 99mTc-HYNIC-cys-AnxV and 99mTc(CO)3-DTPA-cys-AnxV. [Copyright &y& Elsevier]