학술논문

The role of proteins of the outer membrane of Shewanella oneidensis MR-1 in the formation and stabilization of silver sulfide nanoparticles.
Document Type
Article
Source
Applied Biochemistry & Microbiology. Dec2016, Vol. 52 Issue 8, p769-775. 7p.
Subject
*CYTOCHROMES
*SHEWANELLA oneidensis
*SILVER sulfide
*SILVER nanoparticles
*AQUEOUS solutions
*SILVER nitrate
*SODIUM sulfate
Language
ISSN
0003-6838
Abstract
Silver sulfide nanoparticles stable in aqueous solutions were obtained in presence of the cells of the bacterium Shewanella oneidensis MR-1 in aqueous solution containing an equimolar mixture of AgNO and NaSO. Proteins absorbed on the surface of AgS nanoparticles were identified for the first time by MALDITOF/TOF. Among these proteins, multiheme cytochromes MtrC and OmcA, as well as the MtrB membrane porin, which forms a complex on the outer cell membrane, were detected. It was shown that an insoluble precipitate consisting of agglomerated AgS nanoparticles with a wide size distribution was formed in the absence of the cells. The role of the detected proteins in the mechanism of the formation and stabilization of the AgS nanoparticles in the studied system is discussed. [ABSTRACT FROM AUTHOR]