학술논문
Higher rate of central nervous system involvement by flow cytometry than morphology in acute lymphoblastic leukemia.
Document Type
Article
Author
Source
Subject
*CEREBROSPINAL fluid examination
*CENTRAL nervous system diseases
*CYTOLOGY
*FISHER exact test
*FLOW cytometry
*LACTATE dehydrogenase
*LONGITUDINAL method
*LYMPHOBLASTIC leukemia
*PROBABILITY theory
*RECEIVER operating characteristic curves
*DATA analysis software
*MANN Whitney U Test
*DIAGNOSIS
*DISEASE risk factors
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Language
ISSN
1751-5521
Abstract
Introduction Central nervous system ( CNS) involvement in acute lymphoblastic leukemia ( ALL) is diagnosed traditionally by cytopathology ( CP) of the cerebrospinal fluid ( CSF). Role of flow cytometry ( FC) to diagnose CNS involvement has not been extensively investigated. Methods We aimed to detect CNS involvement in 42 ALL patients (33 B- ALL, nine T- ALL) at diagnosis by FC and comparing it with CP and to correlate it with known risk factors for CNS disease like Lactate dehydrogenase ( LDH). A receiver operating characteristic curve was used to determine the cutoff of LDH to predict CSF involvement. For the analysis of categorical/quantitative variables, Fisher's exact test was used. For the analysis of continuous variables, Mann-Whitney test was used. A P value of <.05 was taken as significant. Results CP and FC were positive in five (11.9%) and 11 patients (26.14%) respectively with FC detecting a significantly higher level of involvement ( P=.001). All CP-positive cases were FC positive. A LDH value of >472 U/L had a sensitivity of 61% and specificity of 62.5% for diagnosis of CSF involvement by FC. Conclusions CSF FC detects CNS disease in ALL patients at diagnosis at a rate double than CP alone and is statistically associated with an elevated LDH level. It should be incorporated in the evaluation of CSF to detect CNS involvement. [ABSTRACT FROM AUTHOR]