부산대학교 도서관

Background Vitamin D3 has shown immune-modulating effects in CD4+ T cells from Crohn's disease patients in vitro. Aim To investigate the effects of in vivo vitamin D3 treatment on T cells in Crohn's disease patients. Methods Peripheral blood mononuclear cells (PBMC) were isolated at week 0 and at week 26 from 10 vitamin D3- and 10 placebo-treated Crohn's disease patients participating in a randomized, placebo-controlled, clinical trial study. Monocyte-depleted PBMC were stimulated with anti-CD3 and anti- CD28, and cultured for 7 days, to investigate CD4+ T-cell proliferation and T-cell cytokine production. Results In vitamin D3-treated patients, the median 25-hydroxyvitamin D3 levels increased 70 nmol/L compared with )5 nmol/L in the placebo group. Vitamin D3 treatment increased interleukin-6 production (delta = 188 pg /mL, range: )444 to 4071) compared with a decrease in the placebo group (delta = )896 pg / mL, range: )3841 to 1323) (P < 0.02, Wilcoxon rank sum test). Interestingly, vitamin D3 increased the amount of proliferating stimulated CD4+ T cells from median 41% (range: 10-75%) to 56% (range: 26-77%) (P = 0.02, Wilcoxon rank sum test). Conclusions Vitamin D3 treatment of Crohn's disease patients increased the IL-6 levels. Interestingly, vitamin D3 treatment enhanced the CD4+ T cell proliferation. [ABSTRACT FROM AUTHOR]