학술논문

Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer.
Document Type
Article
Source
Journal of Biomedical Optics. Apr2013, Vol. 18 Issue 4, p1-10. 10p.
Subject
*FLUORESCENCE
*CONFOCAL microscopy
*PRECANCEROUS conditions
*CARCINOMA in situ
*CONFOCAL fluorescence microscopy
Language
ISSN
1083-3668
Abstract
Optical imaging techniques using a variety of contrast mechanisms are under evaluation for early detection of epithelial precancer; however, tradeoffs in field of view (FOV) and resolution may limit their application. Therefore, we present a multiscale multimodal optical imaging system combining macroscopic biochemical imaging of fluorescence lifetime imaging (FLIM) with subcellular morphologic imaging of reflectance confocal microscopy (RCM). The FLIM module images a 16 x 16 mm² tissue area with 62.5 µ m lateral and 320 ps temporal resolution to guide cellular imaging of suspicious regions. Subsequently, coregistered RCM images are acquired at 7 Hz with 400 µ m diameter FOV, <1 µ m lateral and 3.5 µ m axial resolution. FLIM-RCM imaging was performed on a tissue phantom, normal porcine buccal mucosa, and a hamster cheek pouch model of oral carcinogenesis. While FLIM is sensitive to biochemical and macroscopic architectural changes in tissue, RCM provides images of cell nuclear morphology, all key indicators of precancer progression. [ABSTRACT FROM AUTHOR]