부산대학교 도서관

Simple Summary: The isolation and identification of microbes from cancerous lesions are a technical challenges but can contribute to diagnosing the etiology of cervical neoplastic disease. Massive sequencing techniques have facilitated the characterization of microbial communities in the cervix, and the culturomics approach complements the understanding of the mechanistic interactions between microbes and their host. This pilot work aimed to cultivate the cervical microbiota of women with cervical cancer and women without cervical cancer, using media culture conditions available in a bacteriology laboratory coupled with high-performance identification techniques, and to characterize the differences in the microbial composition between both groups. The finding led to a graphical model of hypothetical interactions, indicating changes in cervical microbiota composition, and highlighting some groups of microorganisms relevant to the micro-ecosystem interaction dynamics during each epithelium stage. Thus, the present data provide functional aspects related to living microorganisms that had been scarce in the Mexican population. Traditional microbiological methodology is valuable and essential for microbiota composition description and microbe role assignations at different anatomical sites, including cervical and vaginal tissues; that, combined with molecular biology strategies and modern identification approaches, could give a better perspective of the microbiome under different circumstances. This pilot work aimed to describe the differences in microbiota composition in non-cancer women and women with cervical cancer through a culturomics approach combining culture techniques with Vitek mass spectrometry and 16S rDNA sequencing. To determine the possible differences, diverse statistical, diversity, and multivariate analyses were applied; the results indicated a different microbiota composition between non-cancer women and cervical cancer patients. The Firmicutes phylum dominated the non-cancer (NC) group, whereas the cervical cancer (CC) group was characterized by the predominance of Firmicutes and Proteobacteria phyla; there was a depletion of lactic acid bacteria, an increase in the diversity of anaerobes, and opportunistic and non-typical human microbiota isolates were present. In this context, we hypothesize and propose a model in which microbial composition and dynamics may be essential for maintaining the balance in the cervical microenvironment or can be pro-oncogenesis microenvironmental mediators in a process called Ying-Yang or have a protagonist/antagonist microbiota role. [ABSTRACT FROM AUTHOR]