학술논문
Tyramide signal amplification coupled with multiple immunolabeling and RNAScopein situ hybridization in formaldehyde‐fixed paraffin‐embedded human fetal brain.
Document Type
Article
Source
Subject
*NUCLEIC acid hybridization
*IN situ hybridization
*FETAL brain
*PROTEIN expression
*TELENCEPHALON
*
*
*
*
Language
ISSN
0021-8782
Abstract
Several strategies have been recently introduced to improve the practicality of multiple immunolabeling and RNA in situ hybridization protocols. Tyramide signal amplification (TSA) is a powerful method used to improve the detection sensitivity of immunohistochemistry. RNAScope is a novel commercially available in situ hybridization assay for the detection of RNA expression. In this work, we describe the use of TSA and RNAScope in situ hybridization as extremely sensitive and specific methods for the evaluation of protein and RNA expression in formaldehyde‐fixed paraffin‐embedded human fetal brain sections. These two techniques, when properly optimized, were highly compatible with routine formaldehyde‐fixed paraffin‐embedded tissue that preserves the best morphological characteristics of delicate fetal brain samples, enabling an unparalleled ability to simultaneously visualize the expression of multiple protein and mRNA of genes that are sparsely expressed in the human fetal telencephalon. [ABSTRACT FROM AUTHOR]