부산대학교 도서관

Advances in single-cell technologies have transformed the ability to identify the individual cell types present within tissues and organs. The musculoskeletal bionetwork, part of the wider Human Cell Atlas project, aims to create a detailed map of the healthy musculoskeletal system at a single-cell resolution throughout tissue development and across the human lifespan, with complementary generation of data from diseased tissues. Given the prevalence of musculoskeletal disorders, this detailed reference dataset will be critical to understanding normal musculoskeletal function in growth, homeostasis and ageing. The endeavour will also help to identify the cellular basis for disease and lay the foundations for novel therapeutic approaches to treating diseases of the joints, soft tissues and bone. Here, we present a Roadmap delineating the critical steps required to construct the first draft of a human musculoskeletal cell atlas. We describe the key challenges involved in mapping the extracellular matrix-rich, but cell-poor, tissues of the musculoskeletal system, outline early milestones that have been achieved and describe the vision and directions for a comprehensive musculoskeletal cell atlas. By embracing cutting-edge technologies, integrating diverse datasets and fostering international collaborations, this endeavour has the potential to drive transformative changes in musculoskeletal medicine. A single-cell atlas of the human musculoskeletal system could help to improve the understanding of musculoskeletal function in growth, homeostasis, ageing and disease. This Roadmap delineates the steps required and challenges involved in creating such an atlas. Key points: Musculoskeletal diseases present a large, and growing, global burden and delivery of effective treatments for these diseases requires improved understanding of the cellular basis of musculoskeletal tissue health and disease. The advent of single-cell sequencing methods allows us to build a 'human cell atlas' that identifies and maps every cell of the human body. The application of single-cell technologies in the study of musculoskeletal tissue has historically been difficult owing to the low cellularity and extracellular matrix-rich composition of these tissues. Advances in laboratory and computational methodologies and sharing of knowledge and data among the research community should make it possible to deliver a musculoskeletal cell atlas. Future expansion of the atlas, incorporating a greater number of ancestrally diverse donors, more tissue types and life stages, and diseased samples, will help to accelerate the development of improved diagnostic and therapeutic capabilities. [ABSTRACT FROM AUTHOR]