부산대학교 도서관

Abstract  Trypanosoma cruzicausal agent of Chagas’ disease is a paninfective parasite of mammals transmitted through skin fecal contamination by Triatominae vectors. Studies of alternative routes for infection are scarce; therefore, eye infection should be important, because of the eye’s high blood irrigation and brain proximity, as port of entry of the parasite.Trypanosoma cruziparasites and/or their genetic material in ocular and adjacent muscle tissues were studied in batches of six NMRI mice (15 g) andTrichomys apereoides, an ancient caviomorph (250 g) inoculated withT. cruzimetacyclics from Brazilian (2) and Venezuelan (3) isolates genetically typified asT. cruziI and II. Two animals/batch in the acute or chronic phase were killed and necropsies of cardiac and skeletal muscles, eyeball, and surrounding ocular muscle were processed for hematoxylin–eosine staining. Tissue parasitism was determined. DNA of the digested sections of the eyeball (5–10 μm) was extracted forT. cruzik-DNA amplification by PCR, with S35 and S36 primers. The PCR products were analyzed. The average of maximum values of parasitemia of all infected animals was of 105trypomastigotes/ml blood. Skeletal muscle and heart were colonized in patent infection for all isolates. Amastigote nests were found in corneal tissue of 2/3 of the used isolates and adjacent ocular muscle and connective tissue were parasitized.Trypanosoma cruzik-DNA (330-bp band) was observed in ocular tissue of 4/6 of the isolates studied in both animal models. Investigations concerning infection of the eye globe tissues byT. cruziare extremely scarce. The presence of stages ofT. cruziand/or its genetic products in ocular tissues indicate a broad colonization from a systemic infection. The results show the ocular environment as a possible appropriate microniche forT. cruziand emphasize the risk of transmittingT. cruziby ocular fluids and by parasitized cornea through transplants. [ABSTRACT FROM AUTHOR]