학술논문
Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box.
Document Type
Article
Author
Source
Subject
*SINGLE molecules
*DIMERIZATION
*STOICHIOMETRY
*TRANSCRIPTION factors
*MATERIAL plasticity
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*
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Language
ISSN
1661-6596
Abstract
Sox2 is a pioneer transcription factor that initiates cell fate reprogramming through locus-specific differential regulation. Mechanistically, it was assumed that Sox2 achieves its regulatory diversity via heterodimerization with partner transcription factors. Here, utilizing single-molecule fluorescence spectroscopy, we show that Sox2 alone can modulate DNA structural landscape in a dosage-dependent manner. We propose that such stoichiometric tuning of regulatory DNAs is crucial to the diverse biological functions of Sox2, and represents a generic mechanism of conferring functional plasticity and multiplicity to transcription factors. [ABSTRACT FROM AUTHOR]