부산대학교 도서관

In plants, members of the large PP2C gene family regulate development, growth, and responses to stress exposure. While the tobacco genome is now readily available, there have been few analyses regarding the evolution, expression, and function of PP2C family genes in tobacco plants. Herein, a genome-wide identification strategy was used to explore PP2C gene structures, motifs, and expression patterns in different Nicotiana tabacum L. tissues, with their expression further being evaluated via real-time PCR under a range of stress conditions. In total, 183 NtPP2C genes were identified within the tobacco genome, and phylogenetic analyses revealed these genes to harbor similar gene structures and conserved motifs, and to be clustered within the same clade. The tissue-specific expression patterns of NtPP2C genes from Clade A were additionally characterized. Notably, NtPP2C11 and NtPP2C12 exhibited the highest levels of expression, while NtPP2C18 and NtPP2C19 transcripts were largely undetectable. Specific NtPP2C genes were found to be upregulated in response to low temperature, salt, and drought treatment conditions. Of these, NtPP2C11 and NtPP2C12 were significantly upregulated in response to these treatments, suggesting that they may play important roles in regulating plant responses to exogenous stressors. Overall, these data provide a robust foundation for further functional analyses of NtPP2C genes in tobacco. [ABSTRACT FROM AUTHOR]