학술논문
A NEW MONOMER Ce(III) COMPLEX BASED ON BIS[(2-PYRIDYL)METHYLENE]PYRIDINE- 2,6-DICARBOHYDRAZONE: SYNTHESIS, DNA BINDING, APOPTOSIS, AND MOLECULAR DOCKING.
Document Type
Article
Author
Source
Subject
*MOLECULAR docking
*ULTRAVIOLET spectroscopy
*DNA
*BASE pairs
*X-ray powder diffraction
*MONOMERS
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Language
ISSN
0022-4766
Abstract
A new type of monomer complex [Ce3(L)2(NO3)5(DMF)2] was successfully synthesized by hydrothermal method. The structure of the complex was analyzed by single crystal X-ray diffraction, elemental analysis, and infrared spectroscopy. And the powder X-ray diffraction analysis was used to verify the purity of the complex. UV-Vis (Ultraviolet-visible) absorption spectroscopy shows that the complex is inserted into FS-DNA (fish sperm DNA) through the interaction with DNA base pairs via hydrogen bonds, and the binding constant Kb of DNA is 9.8·104 M–1. Fluorescence spectroscopy experiments revealed the binding capacity of DNA, and the fluorescence quenching constant Ksv value was 0.072·105 M–1. Molecular docking studies have shown that the complex can be effectively inserted into the small groove of DNA. Flow cytometry analyzed the ability of the complex to induce apoptosis HeLa cells (human cervical cancer cells). This series of experiences demonstrated the potential therapeutic value of this complex. [ABSTRACT FROM AUTHOR]