부산대학교 도서관

Simple Summary: Transport stress damages the body health and reduces the immunity of animals. Currently, drugs such as vitamins and antibiotics, etc., are used to alleviate transport stress. In this experiment, lambs were fed diets with electrolytic multivitamin and neomycin, respectively. The weight, stress hormones and immune indicators of sera were examined. At the same time, the villus height, crypt depth and the ratio of villus height to crypt depth (V/C) were measured. Furthermore, the mRNA expressions of Occludin and MUC1, and the protein expression of Occludin in the jejunal mucosa, as well as the mRNA expressions of ZO-1 and Occludin and the protein expression of Occludin in the colonic mucosa were examined. Otherwise, the mRNA expressions of TRAF6, TLR4, MyD88 and NF-kB, and the protein expressions of TLR4 and NF-kB p65, as well as the mRNA expressions of TRAF6, TLR4 and NF-kB in the colon were measured. Adding 375 mg/d/lamb electrolytic multivitamin in the basal diet from 2 d before transportation to 7 d after transportation could potentially improve the immunity and intestinal barrier function. This provides a reference for the application of anti-stress additives to alleviate transport stress of lambs. Animals experience stress when they are transported. In this experiment, sixty 4-month-old lambs were randomly divided into three groups: CG (basal diet), EG (basal diet + 375 mg/d/lamb electrolytic multivitamin) and NG (basal diet + 200 mg/d/lamb neomycin). The transportation day was recorded as the 0th day. Blood, liver, spleen, jejunum and colon were collected on the 0th, 7th and 14th day. The results were as follows: In EG and NG groups, the lamb weights (p < 0.01), IgA and IgG (p < 0.05) increased significantly. The concentrations of ACTH, E, COR, IL-1β, IL-6 and IFN-γ decreased significantly (p < 0.01). The content of colonic propionate increased significantly (p < 0.05). The villus height and V/C increased, and crypt depth decreased significantly (p < 0.01). The mRNA expressions of Occludin and MUC1, and the protein expression of Occludin in the jejunal mucosa, the mRNA expressions of ZO-1 and Occludin, and the protein expression in the colonic mucosa increased significantly (p < 0.01). The mRNA expression of TRAF6 and the protein expression of TLR4 in the jejunum decreased significantly (p < 0.05), as well as the mRNA expressions of TLR4, MyD88 and NF-kB, and the protein expression of NF-kB p65 and the mRNA expressions of TRAF6, TLR4 and NF-kB in the colon (p < 0.01). In conclusion, an electrolytic multivitamin could potentially improve the immunity and intestinal barrier function, and when it was added with 375 mg/d in the basal diet for each lamb from 2 d before transportation to 7 d after transportation, it had a better effect than neomycin. [ABSTRACT FROM AUTHOR]