학술논문
Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study.
Document Type
Article
Author
Trugilo, Kleber Paiva; Cebinelli, Guilherme Cesar Martelossi; Pereira, Érica Romão; Okuyama, Nádia Calvo Martins; Cezar-dos-Santos, Fernando; Castilha, Eliza Pizarro; Flauzino, Tamires; Hoch, Valéria Bumiller-Bini; Watanabe, Maria Angelica Ehara; Guembarovski, Roberta Losi; de Oliveira, Karen Brajão
Source
Subject
*TRANSFORMING growth factors-beta
*HAPLOTYPES
*PROTEIN structure
*PAPILLOMAVIRUSES
*CASE-control method
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Language
ISSN
2073-4409
Abstract
This study aimed to verify the role of TGFB1 variants (c.–1638G>A, c.–1347C>T, c.29C>T, and c.74G>C) in HPV infection susceptibility and cervical lesions development, and their impact on TGFB1 cervical and plasma levels. TGFB1 genotypes were assessed with PCR-RFLP and haplotypes were inferred for 190 HPV-uninfected and 161 HPV-infected women. TGFB1 levels were determined with immunofluorimetric assay. Case-control analyses were performed with logistic regression adjusted for possible confounders. Women carrying –1347TT or –1347CT+TT as well as those with 29CT, 29CC, or 29CT+CC were more likely to have HPV than –1347CC and 29TT carriers, respectively. Regarding haplotypes, the most frequent were *4 (GCTG) and *3 (GTCG). Women *4/*4 were less likely to have HPV than those with no *4 copy. Comparing the inheritance of *3 and *4, carriers of *3/*4 or *3/*3 were more susceptible to HPV than *4/*4. The TGFB1 plasma and cervical levels were higher in the infected patients. Plasma levels were also higher in infected women with low-grade lesions. HPV-infected patients carrying *3/Other and *3/Other+*3/*3 presented lower TGFB1 plasma levels than those with no copy of *3. TGFB1 variants could contribute to the comprehension of the TGFB1 role in HPV-caused cervical disease. [ABSTRACT FROM AUTHOR]