학술논문
POLYIMIDAZOLE CONJUGATED OLIGONUCLEOTIDES REACH THE NUCLEUS OF HELA CELLS.
Document Type
Article
Source
Subject
*OLIGONUCLEOTIDES
*HISTAMINE
*BIOSYNTHESIS
*FLUORESCEIN
*HELA cells
*
*
*
*
Language
ISSN
1525-7770
Abstract
Oligonucleotide models bearing 6, 12 or 18 histamine residues were synthesized on solid support and labeled with fluorescein. Only the oligo with 6 histamine residues showed a high uptake in HeLa cells with a nuclear localization. Experiment a 4°C or with bafilomicyn A1 suggest that uptake proceeded by an endocytosis mechanism followed by a destabilization of the membrane. Once in the cytoplasm the oligo reached rapidly the nucleus. [ABSTRACT FROM AUTHOR]