학술논문
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
Document Type
Article
Author
Source
Subject
*CHROMATIN
*CHROMOSOMES
*PROTEINS
*SPINDLE apparatus
*MICROTUBULES
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Language
ISSN
0028-0836
Abstract
Shows that by using the chromatin bead assay the activity of chromosome-associated RCC1 protein is required for spindle formation. Induction of microtubule nucleation and spindle-like structures in M-phase extract through Ran, when in GTP-bound state (Ran-GTP); Proposal that RCC1 generates a high local concentration of Ran-GTP around chromatin, which in turn induces the local nucleation of microtubules.