부산대학교 도서관

In general, QuantAS provides an accurate quantitative method for isoform quantification, reduces systematic errors in experiments, and demonstrates high compatibility and specificity, while other methods, such as HiFENS, based on Fluorescence I in situ i hybridization (FISH) technique for endogenous splicing isoforms detection, which still relies on isoform-specific regions (Shilo I et al i ., [21]). Keywords: absolute quantification; alternative splicing; digital PCR; QuantAS; quantitative real-time PCR EN absolute quantification alternative splicing digital PCR QuantAS quantitative real-time PCR 928 939 12 10/09/23 20231101 NES 231101 Data availability All data supporting the findings are contained in this manuscript and Supporting Information. The method utilizes isoform-specific primers to overcome the isoform identification difficulty caused by different AS events and is designed by using the functional coding region as the isoform structure classification unit to ensure isoform independence (Fig. [Extracted from the article]