학술논문
Detection and Quantification of Protein–Microtubules Interactions Using Green Fluorescent Protein Photoconversion.
Document Type
Article
Author
Source
Subject
*GREEN fluorescent protein
*RECOMBINANT proteins
*TUBULINS
*ACTIN
*CELL motility
*MITOSIS
*FLUORESCENCE microscopy
*
*
*
*
*
*
Language
ISSN
1398-9219
Abstract
We present an in vitro system to analyze quantitatively the interactions of green fluorescent protein (GFP)-tagged recombinant proteins with microtubules. This method relies on photoconversion of GFP and time-lapse microscopy. Specific interactions can be detected and binding kinetics can be determined rapidly and accurately. This method provides an alternative to classical in vitro microtubule-binding assays to analyze microtubule-associated proteins binding to microtubules. It has the potential to be extended to study interactions of proteins or multi-protein complexes with different biopolymers like actin microfilaments or organelle membranes. [ABSTRACT FROM AUTHOR]