부산대학교 도서관

Simple Summary: In scientific research, there are various fields that require the simultaneous sampling of tissue and marking of mice shortly after birth. One approach is the amputation of the distal phalanx, a method that not only provides a lasting mark but also allows for the use of the removed tissue for early genotyping. While the use of this method is a topic of ongoing debate, it remains one of the safest and most widely employed techniques in current practice. The key lies in practical experience, ensuring the correct application of the method. To enhance and optimize this process, we have developed a 3D dummy specifically designed for training individuals in the precise amputation of the distal phalanx in 5-day-old mouse pups. In a recent seminar, we sought to evaluate the effectiveness of training on this innovative model and assess its appropriateness for the task at hand. The development of realistic dummies for training the distal phalanx amputation (DPA) technique in mouse pups is a promising alternative to reduce and replace animals in training for research and teaching. To test this, we obtained micro-CT data from postnatal day-five mouse pups, meticulously segmented them, and converted them into a 3D mesh format suitable for 3D printing. Once the dummy was printed, it was evaluated during actual training courses in two different groups: in the first group, users received no dummies to train the DPA, and in the second group, users were trained with three dummies. To assess the effectiveness of the dummy, we conducted a survey followed by an expert veterinarian evaluation. Our results showed that DPA is a complex procedure, and it is commonly poorly performed. When implementing the dummies, users who were not provided with dummies to practice only had an 8.3% success rate in DPA, while users provided with three dummies had a 45.5% success rate, respectively. Despite additional research being needed, our dummy offered improved practical training by providing a safe and effective alternative in line with ethical considerations while demonstrating the feasibility of using 3D printing technology to promote the 3Rs in experimental research. [ABSTRACT FROM AUTHOR]