학술논문
Genome-Wide Binding of MBD2 Reveals Strong Preference for Highly Methylated Loci.
Document Type
Article
Author
Source
Subject
*DNA methylation
*LOCUS (Genetics)
*METHYL-CpG-binding protein 2
*GENETIC repressors
*GENE expression
*PROMOTERS (Genetics)
*COHORT analysis
*BREAST cancer
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Language
ISSN
1932-6203
Abstract
MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. In this study we adopted an MBD2 tagging-approach to study its genome wide binding characteristics. We show that in vivo MBD2 is mainly recruited to CpG island promoters that are highly methylated. Interestingly, MBD2 binds around 1 kb downstream of the transcription start site of a subset of ∼400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. These tagged-MBD2 binding sites in MCF-7 show increased methylation in a cohort of primary breast cancers but not in normal breast samples, suggesting a putative role for MBD2 in breast cancer. [ABSTRACT FROM AUTHOR]