부산대학교 도서관

Mast cell (MC) heterogeneity has been well characterized in the rat where it has been shown that connective tissue MC, often represented by peritoneal MC (PMC), and the intestinal mucosal MC (IMMC) exhibit many differences in mediator content and responsiveness to secretagogues and anti-allergic drugs. Pretreatment (20 hr) of PMC with interferon (IFN)-α/β or IFN-γ, significantly reduced antigen-stimulated histamine release. By contrast, for IMMC, the same IFN treatment did not modify antigen-stimulated histamine secretion. Although IFN treatment differentially modulates histamine secretion from PMC and IMMC, pretreatment of both MC types with IFN-α/β or IFN-γ inhibited their tumour-necrosis factor-α (TNF-α)-dependent cytotoxicity. In 20 hr of culture, IMMC spontaneously released 98pg/106 MC of TNF-α, whereas PMC released about threefold more TNF-α, 282pg/106 MC. In addition, direct assessment of stored TNF-α established that IMMC store less TNF-α (68pg/106 MC) than PMC (404 pg/106 MC). In summary, TNF-α content of PMC and IMMC was different, but IFN inhibited TNF-α-dependent cytotoxicity by both MC types. By contrast, treatment with IFN-α/β or IFN-γ inhibits antigen-induced histamine secretion by PMC, but does not modify antigen-induced histamine secretion by IMMC. Thus, IFN differentially regulate the secretion of histamine and TNF-α in PMC and IMMC. [ABSTRACT FROM AUTHOR]