학술논문
Differentiation of mouse embryonic stem cells into a defined neuronal lineage.
Document Type
Article
Author
Source
Subject
*EMBRYONIC stem cells
*STEM cells
*NERVOUS system
*TRETINOIN
*DERMATOLOGIC agents
*NEUROGLIA
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*
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Language
ISSN
1097-6256
Abstract
Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics. [ABSTRACT FROM AUTHOR]