부산대학교 도서관

The disease huanglongbing (HLB), caused by tire phloem-limited and psyllid-vectored 'Candidatus Li-beribacter' spp., is threatening tire Mediterranean citrus industry. The African psyllid (Trioza erytreae) vector of tire patlrogen was detected in Madeira (Portugal) in 1994 and in tire Canary Islands (Spain) in 2002, and its arrival in 2014 in northwest Spain and Portugal along tire Atlantic coast instigated a biological alert, and a contingency man-agement plan was developed. Extensive surveys were conducted in Canarv Islands fronr 2009 to 2015 and in tire northwest mainland Spain (Galicia) since the first detection of T. erytreae. Symptoms of the psyllid were observed in most sweet orange orchards of five islands in C air ary Islairds (93% of the iirspected plots). In northwest nrain-land Spain, 65% of the iirspected plots up to 2016 showed T. erytreae symptoms. During tire surveys, ten leaves/tree from trees showing suspicious symptoms and from symptomless trees, as well as adult psyllids, were collected and analysed by real-time PCR using a universal 'Ca. Liberibacter' spp. kit, according to tire EPPO standard. Suspected samples from other surveyed Spanish regions free of the vector were also analysed. The few samples that were positive in the screening test were tested by species-specific real-time PCR protocols, and they did not show amplification. These data confirm that the Spanish citrus industry is currently free of the 'Ca. Liberibacter' spp., but strict measures to prevent tire introduction of this patlrogen are required as tire presence of T. erytreae increases the risk of its dissemination. [ABSTRACT FROM AUTHOR]