학술논문
Recombinant Production and Structure–Function Study of the Ts1 Toxin from the Brazilian Scorpion Tityus serrulatus.
Document Type
Article
Author
Source
Subject
*TITYUS
*GENE expression
*SCORPIONS
*TOXINS
*VOLTAGE-gated ion channels
*NUCLEAR magnetic resonance spectroscopy
*ESCHERICHIA coli
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Language
ISSN
1607-6729
Abstract
An effective bacterial system for the production of β-toxin Ts1, the main component of the Brazilian scorpion Tityus serrulatus venom, was developed. Recombinant toxin and its 15N-labeled analogue were obtained via direct expression of synthetic gene in Escherichia coli with subsequent folding from the inclusion bodies. According to NMR spectroscopy data, the recombinant toxin is structured in an aqueous solution and contains a significant fraction of β-structure. The formation of a stable disulfide-bond isomer of Ts1, having a disordered structure, has also been observed during folding. Recombinant Ts1 blocks Na+ current through NaV1.5 channels without affecting the processes of activation and inactivation. At the same time, the effect upon NaV1.4 channels is associated with a shift of the activation curve towards more negative membrane potentials. [ABSTRACT FROM AUTHOR]