학술논문
Phenolic Composition and Anti-acetylcholinesterase Activity of Two Euphrasia rostkoviana Hayne Species from Different Localities.
Document Type
Abstract
Author
Source
Subject
*EUPHRASIA
*PHENOL
*ACETYLCHOLINESTERASE inhibitors
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Language
ISSN
0367-4444
Abstract
Euphrasia rostkoviana Hayne is a hemi-parasitic plant from genus Euphrasia which has been used in traditional medicine for treatment of various eye diseases and conditions. In spite of its use, little has been confirmed and published regarding the biological activity and chemical composition of this plant species. Thus, we investigated phenolic composition of hydromethanolic extracts of Euphrasia rostkoviana from two different locations in Serbia and their ability to inhibit acetylcholinesterase enzyme. Out of 70 phenolic compounds analyzed by LC-MS/MS technique, 36 compounds were detected. The extracts were mainly composed of flavones and phenolics acids. Great differences have been observed between the samples obtained from different localities, which was particularly noticeable in quantities of chlorogenic acid, rutin, quinic acid, caffeic acid and luteolin-7-O-glucoside. The extracts exhibited similar ability to inhibit acetylcholinesterase (IC50=3.49 mg/mL and IC50=2.78 mg/mL) but in comparison to standard galantamine (IC50=0.1 μg/mL) the activity was weak. This study pointed out that Euphrasia rostkoviana is a rich source of phenolic compounds but its composition varies greatly, probably due to the plant's hemiparasitic nature. Nonetheless, it is possible that quantities of compounds responsible for the extract's anti-acetylcholinesterase activity are consistent. [ABSTRACT FROM AUTHOR]