부산대학교 도서관

Potato (Solanum tuberosum) is the most consumed non-cereal food crop. Most commercial potato cultivars are autotetraploids with highly heterozygous genomes, severely hampering genetic analyses and improvement. By leveraging the state-of-the-art sequencing technologies and polyploid graph binning, we achieved a chromosome-scale, haplotype-resolved genome assembly of a cultivated potato, Cooperation-88 (C88). Intra-haplotype comparative analyses revealed extensive sequence and expression differences in this tetraploid genome. We identified haplotype-specific pericentromeres on chromosomes, suggesting a distinct evolutionary trajectory of potato homologous centromeres. Furthermore, we detected double reduction events that are unevenly distributed on haplotypes in 1021 of 1034 selfing progeny, a feature of autopolyploid inheritance. By distinguishing maternal and paternal haplotype sets in C88, we simulated the origin of heterosis in cultivated tetraploid with a survey of 3110 tetra-allelic loci with deleterious mutations, which were masked in the heterozygous condition by two parents. This study provides insights into the genomic architecture of autopolyploids and will guide their breeding. We achieved a chromosome-scale, haplotype-resolved genome assembly of a widely grown potato variety, Cooperation-88 (C88), with the combined state-of-the-art sequencing technologies and polyploid graph binning. Extensive differences on pericentromeric sequence and gene expression, and uneven double reduction landscape were revealed within four haplotypes, which shaped the genomic architecture of the cultivated potato. Masking of heterozygous deleterious mutations and accumulation of functional alleles contributed the fine commodity properties of C88. [ABSTRACT FROM AUTHOR]