학술논문
Estimated copy number of Bacillus anthracis plasmids pXO1 and pXO2 using digital PCR
Document Type
Article
Author
Source
Subject
*BACILLUS anthracis
*POLYMERASE chain reaction
*CHROMOSOME duplication
*PLASMID genetics
*NUCLEOTIDE sequence
*COMPARATIVE studies
*QUANTITATIVE research
*MICROBIOLOGICAL techniques
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Language
ISSN
0167-7012
Abstract
Abstract: We evaluated digital PCR (dPCR) to directly enumerate plasmid and chromosome copies in three strains of Bacillus anthracis. Copy number estimates based on conventional quantitative PCR (qPCR) highlighted the variability of using qPCR to measure copy number whereas estimates based on direct sequencing are comparable to dPCR. [Copyright &y& Elsevier]