부산대학교 도서관

Resveratrol is a plant-derived phytoalexin with antioxidant, anti-inflammatory and cardio-protective properties and may be a promising therapeutic intervention strategy in cardiovascular disease. Here, we investigated the acute direct effects of trans-resveratrol (RV), on acetylcholine (ACh)-induced and flow-mediated dilation (FMD) of isolated pressurized femoral arteries of young (4-month-old) and old (26-month-old) mice. Vessel exposure to RV enhanced ACh (0.01–1.0 mM)-induced dilation (p < 0.05), but not FMD (@ 5–10 μL⋅min−1) (p < 0.05) in both young and old mice. After RV incubation, acute nitric oxide (NO) production by cultured endothelial cells was increased in response to 0.01 mM ACh, but reduced by flow (5–10 μL⋅min−1; p < 0.05). In isolated femoral arteries from endothelial nitric oxide synthase knockout (eNOS−/-) mice, RV had no overall effect on FMD, but potentiated ACh induced dilation, that was completely abolished by potassium channel blockers, Apamin and Tram 34 (p < 0.01). We demonstrate that the non-metabolised form of RV stimulates ACh-induced dilation via the NO and EDHF pathways, but not FMD by interaction with the cyclo-oxygenase pathway. Our findings have important implications in the use of RV (for both young and aged) under 'normal' non-diseased physiological states. • Resveratrol may be a promising therapy in cardiovascular disease and ageing. • We examined direct effects of resveratrol on femoral artery dilation in mice. • Resveratrol enhances acetylcholine-induced, but not flow mediated dilation. • Resveratrol stimulates ACh-induced dilation via the NO and EDHF pathways. • Femoral dilator effects depend on nature of stimulus in young and aged mice. [ABSTRACT FROM AUTHOR]