부산대학교 도서관

This study involves the function prediction of hypothetical proteins found in the genome Pseudomonas putida. About 20 hypothetical proteins were screened for domains of unknown functions. Eleven DUFs were selected for the study. The function was predicted based on cellular localization and homology structure modelling using bioinformatics tools Signal P, Target P, HHPred and CPHpred. The hypothetical genes containing domains with unknown function (DUF) from the genome of plant endophytic bacterial strain Pseudomonas putida were chosen. Results Out of 11 DUF genes selected for the study protein structure was predicted for the domain of unknown function DUF3530. The localization and presence of signal peptide were predicted for all the DUFs. The homology model predicted was selected based on the Z-score (7.7) and E-value (7.8e-19). Conclusion The predicted structure was found to be similar to a carboxyl ester hydrolase in Bacillus coagulans bacteria. [ABSTRACT FROM AUTHOR]