학술논문

A genome-wide 3C-method for characterizing the three-dimensional architectures of genomes
Document Type
Article
Source
Methods. Nov2012, Vol. 58 Issue 3, p277-288. 12p.
Subject
*GENOMES
*CHROMOSOMES
*CELL nuclei
*DNA replication
*DNA repair
*GENETIC transcription
Language
ISSN
1046-2023
Abstract
Abstract: Accumulating evidence demonstrates that the three-dimensional (3D) organization of chromosomes within the eukaryotic nucleus reflects and influences genomic activities, including transcription, DNA replication, recombination and DNA repair. In order to uncover structure-function relationships, it is necessary first to understand the principles underlying the folding and the 3D arrangement of chromosomes. Chromosome conformation capture (3C) provides a powerful tool for detecting interactions within and between chromosomes. A high throughput derivative of 3C, chromosome conformation capture on chip (4C), executes a genome-wide interrogation of interaction partners for a given locus. We recently developed a new method, a derivative of 3C and 4C, which, similar to Hi-C, is capable of comprehensively identifying long-range chromosome interactions throughout a genome in an unbiased fashion. Hence, our method can be applied to decipher the 3D architectures of genomes. Here, we provide a detailed protocol for this method. [Copyright &y& Elsevier]