학술논문
Effect of Conjugated Linoleic Acid-enriched Butter After 24 hours of Intestinal Mucositis Induction.
Document Type
Article
Author
Barros, Patrícia Aparecida Vieira de; Generoso, Simone de Vasconcelos; Andrade, Maria Emília Rabelo; da Gama, Marco Antonio Sundfeld; Lopes, Fernando Cesar Ferraz; de Sales e Souza, Éricka Lorenna; Martins, Flaviano dos Santos; Miranda, Sued Eustáquio Mendes; Fernandes, Simone Odília Antunes; Cardoso, Valbert Nascimento
Source
Subject
*SMALL intestine surgery
*INTESTINAL physiology
*REACTIVE oxygen species
*ANIMAL experimentation
*BODY weight
*BUTTER
*CANCER chemotherapy
*CYTOKINES
*DIET
*DNA
*ENZYME-linked immunosorbent assay
*FLUOROURACIL
*IMMUNOGLOBULINS
*INFLAMMATION
*INGESTION
*INJECTIONS
*MICE
*RADIOTHERAPY
*RESEARCH funding
*LINOLEIC acid
*CONTROL groups
*GASTROINTESTINAL mucosa
*DATA analysis software
*DIAGNOSIS
*DISEASES
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Language
ISSN
0163-5581
Abstract
Mucositis is the most common side effect due to chemotherapy or radiotherapy. It refers to the inflammation of intestinal mucous membranes, and it is associated with complications such as diarrhea, weight loss, and increased intestinal permeability (IP). This study was designed to evaluate the effect of diet containing conjugated linoleic acid (CLA)-enriched butter on intestinal damage and inflammatory response after 24 h of 5-fluorouracil (5-FU)-induced mucositis. Mice were divided into four groups: CTL; CLA; 5-FU, and CLA 5-FU, and they were fed for 31 days. On the 30th experimental day, mucositis was induced by unique injection of 300 mg/kg of 5-FU. After 24 h (31st experimental day), IP was evaluated; ileum and fecal material were collected to determine cytokine level and myeloperoxidase (MPO) activity and secretory immunoglobulin A (sIgA). The 5-FU group showed an increase in IP and MPO activity (CTL vs. 5-FU:P< 0.05). Additionally, increased levels of IP and MPO were observed in CLA 5-FU group compared to those in the test groups (P< 0.05). Animals in the CLA 5-FU group showed reduced concentrations of sIgA (CTL vs. CLA 5-FU:P< 0.05). CLA-enriched butter exacerbating the 5-FU-induced intestinal damage. Safety concerns regarding the use of CLA require further investigation. [ABSTRACT FROM PUBLISHER]