학술논문
Cloning and characterization of the tomato karyopherinα1 gene promoter.
Document Type
Article
Source
Subject
*PROMOTERS (Genetics)
*CLONING
*PLANT genetic engineering
*GENE expression
*TRANSGENIC plants
TOMATO genetics
*
*
*
*
Language
ISSN
0012-1592
Abstract
The karyopherinα1 (LeKAPα 1) gene of tomato (Lycopersicon esculentum) encodes a receptor involved in nuclear import. To analyze the expression pattern of this gene, a genomic clone containing its upstream region was isolated and sequenced. To study the promoter functionality, a 2170 bp fragment (LM1), was fused to glucuronidase (GUS) and introduced into petunia cells by particle bombardment. For further characterization of the promoter, one inverse and three deletion constructs were studied in cell suspension. To follow its expression in tobacco leaves, transgenic plants expressing GUS under the control of the LM1 promoter were made. Expression of LM1–GUS was largely restricted to actively growing leaf regions, suggesting possible involvement of active cell division and plant growth regulators inLeKAPα 1 expression. [ABSTRACT FROM AUTHOR]