학술논문
Genomic analyses of ciprofloxacin-resistant Neisseria gonorrhoeae isolates recovered from the largest South American metropolitan area.
Document Type
Article
Author
Source
Subject
*NEISSERIA gonorrhoeae
*GENOMICS
*RIFAMPIN
*METROPOLITAN areas
*WHOLE genome sequencing
*TETRACYCLINES
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Language
ISSN
0888-7543
Abstract
We sequenced 13 Neisseria gonorrhoeae isolates exhibiting distinct susceptibility profiles and which were recovered over 12 years in the metropolitan region of São Paulo, Brazil. Whole Genome Sequencing (WGS) was performed on an Illumina MiSeq™ 2 × 300 bp paired-end reads. Bioinformatics analyses were carried out using CGE, PATRIC, and BLAST databases for manual curation of obtained genomes. Multilocus sequence typing (MLST) analysis identified seven STs, namely ST1580, ST1590, ST1901, ST1902, ST8161, ST9363, and ST15640. Moreover, a diversity of mutations was observed in MtrR/G45D-A39T, PIB/G120K-A121S, and PBP1/L421P. Mutations associated with sulfonamides (DHPS/R228S) and rifampicin (RNAP/H552N) were also detected, as well as tetracycline resistance determinants, namely rpsJ /V57M and tet (M). The results presented herein can contribute to the knowledge of N. gonorrhoeae strains circulating in Sao Paulo, Brazil. • We evaluated the resistome of 11 Neisseria gonorrhoeae isolated between 2003 and 2015. • The ST90/CC90 clone emerged in the São Paulo region within the year 2013. • Resistance genes/mutations to five antimicrobial classes were detected. • Despite high MICs for cephalosporins, no ESBL-producing N. gonorrhoeae was seen. [ABSTRACT FROM AUTHOR]