학술논문
Standardization procedure for flow cytometry data harmonization in prospective multicenter studies.
Document Type
Article
Author
Le Lann, Lucas; Jouve, Pierre-Emmanuel; Alarcón-Riquelme, Marta; Jamin, Christophe; Pers, Jacques-Olivier; PRECISESADS Flow Cytometry Study Group; Alvarez, Montserrat; Alvarez-Errico, Damiana; Azevedo, Nancy; Barbarroja, Nuria; Buttgereit, Anne; Cheng, Qingyu; Chizzolini, Carlo; Cremer, Jonathan; De Groof, Aurélie; De Langhe, Ellen; Ducreux, Julie; Dufour, Aleksandra; Gerl, Velia; Hernandez-Fuentes, Maria
Source
Subject
*LEUCOCYTES
*FLOW cytometry
*DATA analysis
*STANDARDIZATION
*MEDICAL centers
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Language
ISSN
2045-2322
Abstract
One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fluorescence intensities of cell markers, especially when the data are generated from different instruments. Here, we describe an innovative standardization workflow to compare all data to carry out any large-scale, prospective multicentric flow cytometry analysis whatever the duration, the number or type of instruments required for the realization of such projects. [ABSTRACT FROM AUTHOR]