학술논문
Enantioselective Quantification of Atenolol in Mouse Plasma by High Performance Liquid Chromatography Using a Chiral Stationary Phase: Application to a Pharmacokinetic Study.
Document Type
Article
Source
Subject
*ATENOLOL
*LABORATORY mice
*BLOOD plasma
*HIGH performance liquid chromatography
*PHARMACOKINETICS
*CHIRAL drugs
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Language
ISSN
1060-3271
Abstract
Enantiomeric resolution of atenolol was achieved on the HPLC vancomycin macrocyclic antibiotic chiral stationary phase Chirobiotic V. The polar ionic mobile phase consisted of methanol--glacial acetic acid--triethylamine (100 + 0.025 + 0.75, v/v/v) at a low rate of 0.8 mL/min. Fluorescence detection at 275/305 nm for excitation and emission, respectively, was used. Plasma samples were purified using SPE on Oasis HLB cartridges. The calibration curves in plasma were linear over the range of 5-400 ng/mL (r = 0.999) for each enantiomer with an LOD of 1.0 ng/mL. The proposed method was validated in compliance with International Conference of Harmonization guidelines in terms of linearity, accuracy, precision, LOD, LOQ, and selectivity. The overall recoveries for S-(-)- and R-(+)-atenolol enantiomers from plasma were 95.0-99.5%; RSD ranged from 2.5 to 3.3%. The developed method was applied for the trace analysis of atenolol enantiomers in plasma and for the pharmacokinetic investigation of atenolol enantiomers in mouse plasma. [ABSTRACT FROM AUTHOR]