부산대학교 도서관

Background: Alzheimer disease (AD) is a progressive neurological degenerative disorder and the most common form of dementia. There are about 100 genes linked to AD including apolipoprotein E (ApoE). This gene exists in the form of three allele polymorphisms of e2, £ 3 and u and six genotypes of £2£3, £2£2, £s£3, £2£4, £s£4, and £4£4. We aimed to study the association of ApoE polymorphism with AD in Guilan province, Iran. Methods: The study group consisted of 70 AD patients and 1 0 0 healthy individuals as a control group. All subjects were recruited from 21 March to 22 September 2015 at Imam Reza Clinic, Rasht, Iran. The genomic deoxyribonucleic acid (DNA) was extracted from peripheral blood leucocytes, and subsequently, subjects were genotyped for ApoE using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The association between the risk allele and AD was assessed using the MedCalc software. Results: The distributions of e3£3, £3£/i, £2£2, £2£4, £4£4 and £2e3 Genotypes among patients were 55.7%, 30.0%, 1.4%, 2.9%, 8 .6 %, 1.4% and in the controls were 79.0%, 8.0%, 0%, 1.0%, 1.0%, 11.0%, respectively. The genotype frequencies were significantly different between cases and the controls (P < 0.001). The individuals with the £4£4 and £ 3£ 4 genotypes had a greater risk for AD as compared to others; odds ratio (OR) = 12.15, 95% confidence interval (Cl): 1.41-104.50, P = 0.020; OR = 5.32,95% Cl: 2.16-13.08, P = 0.003. In addition, the £4 allele is significantly associated with higher AD risk among the studied population (OR = 5.63, 95% Cl: 2.74-11.58, P< 0.001). Conclusion: This case-control study suggests that the subjects with £ 4£ 4 and £ 3£ 4 genotypes had an increased risk for AD in Iranian population. [ABSTRACT FROM AUTHOR]