부산대학교 도서관

Rhipsalis neves-armondii K. Schum. (Cactaceae) aerial parts preparation is used traditionally in southern Nigerian for the treatment of rheumatic disorders and cancer and so the aim of this study was to evaluate these folkloric claims. Carragenaan-induced pedal edema and cotton pellet-induced granuloma were the anti-inflammatory models employed, while the antiploliferative study was performed using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide] assay method on Pancreatic tumor (PANC-1) and Henrietta Lacks' cervical (HeLa) cell lines. The dried pulverized Rhipsalis aerial parts were extracted with methanol to obtain Rhipsalis neves-armondii extract (RNE). The RNE was fractionated using chromatographic techniques to obtain the hexane fraction (HF) and ethylacetate fraction (EF). Also acute toxicity and phytochemical studies were performed using standard procedures. Results showed that the extract and fractions significantly (p < 0.05) inhibited the development of rat pedal edema. The EF (400 mg/kg) exhibited the highest percentage edema inhibition of 53.33% after 3 h, compared to 36.67% of indomethacin. Also the extract and fractions significantly (p < 0.05) suppressed granuloma formation in rats, with HF showing the highest percentage suppression of 59.4% compared to the control. The RNE exhibited potent cytotoxic effect against PANC-1 and HeLa cells with an estimated IC50 value of 30.12 and 45.1 μg/ml, respectively, and showed an LD50 greater than 5,000 mg/kg. The findings showed that the extract and fractions of Rhipsalis neves-armondii (Cactaceae) possess anti-inflammatory and antiproliferative effects. [ABSTRACT FROM AUTHOR]