부산대학교 도서관

Leprosy is still a public health problem in Indonesia especially in the Eastern part of Indonesia. In the midst of our effort to combat leprosy, drug resistance was reported in some endemic areas of Indonesia. Drug resistance surveillance and strain typing of Mycobacterium leprae are necessary to magnitude our effort to eliminate leprosy. gyrA gene is associated with ofloxacin antibiotic, the second line of leprosy treatment after the standard Multi Drugs Therapy (MDT). In this study, we aimed to reveal our findings on the mutations on the gyrA gene of M. leprae from leprosy patients in West Papua and Papua provinces. Bacterial samples were collected from slit skin smear and extracted using Qiaamp mini DNA extraction kit. gyrA amplification was carried out using GoTaq Green Mastermix. Sanger sequencing was done using BigDye Terminator v3.1. Alignment analysis was performed with M. leprae TN as the referral strain. The phylogenetic tree was constructed using Mega 7 to get the M. leprae gyrA cluster. The RNAalifold server was employed to generate the conserved 2D structure for the gyrA multiple sequence alignment (MSAs). Six variants of gyrA M. leprae were found from West Papua and Papua provinces. The six variants are H71R, K73R, D95G, A101T, R107W, A127V. The existence of mutations in the gyrA gene of M. leprae found in this study can be information for the treatment of leprosy in Papua if ofloxacin is used as an alternative treatment. Based on the phylogenetic analysis, there are three distinct clusters of the gyrA gene. The D95G variant has been confirmed to cause resistance to fluoroquinolone in vitro, while the H71R, K73R, A101T, R107W, A127V variants are new variants. Based on the analysis of the mutation impact, the H71R, K73R, A101T, R107W, A127V mutants had moderate impacts. However, an in vivo test is required to validate these results. [ABSTRACT FROM AUTHOR]