학술논문
Comparison of freeze-thaw cycles for nucleic acid extraction and molecular detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in environmental matrices.
Document Type
Article
Source
Subject
*FREEZE-thaw cycles
*NUCLEIC acid analysis
*DETACHMENT reactions
*CRYPTOSPORIDIUM parvum
*TOXOPLASMA gondii
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Language
ISSN
0167-7012
Abstract
Abstract Freeze-thaw DNA extraction methods and PCR primers were compared to optimize detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in different matrices. Increasing FT cycles did not increase parasite DNA detection, and primers targeting the 18S ssrRNA gene yielded the most sensitive detection of C. parvum oocysts. Highlights • Increasing Freeze-thaw (FT) cycles did not enhance parasite DNA extraction. • Toxoplasma gondii was detected in mussel tissue and water following one FT cycle. • Cryptosporidium parvum detection was highest targeting the 18S ssrRNA gene. [ABSTRACT FROM AUTHOR]