부산대학교 도서관

Three recombinant proteins of Echinococcus granulosus including two antigen B sub-units Eg AgB8/1 and Eg AgB8/2 and Echinococcus protoscolex calcium binding protein 1 (EPC1) were expressed in prokaryotic expression vectors. The diagnostic potential of these three recombinant proteins was evaluated in the detection of cystic echinococcosis in buffaloes in IgG-ELISA. The Eg AgB8/1 and Eg AgB8/2 recombinant proteins reacted fairly with the hydatid infected buffaloes with sensitivity of 75.0% and 78.6%, respectively and specificity of 75.8% while EPC1 recombinant protein showed higher sensitivity (89.3%) but lower specificity (51.5%). Cross-reactivity of these three antigens was assayed with buffalo sera naturally infected with Explanatum explanatum, Paramphistomum epiclitum, Gastrothylax spp., Fasciola gigantica and Sarcocystis spp. Eg AgB8/1 and EPC1 antigens cross-reacted with all these sera while Eg AgB8/2 showed no cross-reaction with Sarcocystis spp. and reacted with some of the E. explanatum infected buffalo sera. This study explores the potential of three hydatid antigens viz . Eg AgB8/1, Eg AgB8/2 and EPC1 for their diagnostic potential in buffaloes positive for cystic echinococcosis. [ABSTRACT FROM AUTHOR]