학술논문
Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae.
Document Type
Article
Author
Brabham, Helen J; Gómez De La Cruz, Diana; Were, Vincent; Shimizu, Motoki; Saitoh, Hiromasa; Hernández-Pinzón, Inmaculada; Green, Phon; Lorang, Jennifer; Fujisaki, Koki; Sato, Kazuhiro; Molnár, István; Šimková, Hana; Doležel, Jaroslav; Russell, James; Taylor, Jodie; Smoker, Matthew; Gupta, Yogesh Kumar; Wolpert, Tom; Talbot, Nicholas J; Terauchi, Ryohei
Source
Subject
*PYRICULARIA oryzae
*RICE blast disease
*ERYSIPHE graminis
*POWDERY mildew diseases
*GENETIC testing
*BARLEY
*HORDEUM
*
*
*
*
*
*
Language
ISSN
1040-4651
Abstract
Plant nucleotide-binding leucine-rich repeat (NLRs) immune receptors directly or indirectly recognize pathogen-secreted effector molecules to initiate plant defense. Recognition of multiple pathogens by a single NLR is rare and usually occurs via monitoring for changes to host proteins; few characterized NLRs have been shown to recognize multiple effectors. The barley (Hordeum vulgare) NLR gene Mildew locus a (Mla) has undergone functional diversification, and the proteins encoded by different Mla alleles recognize host-adapted isolates of barley powdery mildew (Blumeria graminis f. sp. hordei [Bgh]). Here, we show that Mla3 also confers resistance to the rice blast fungus Magnaporthe oryzae in a dosage-dependent manner. Using a forward genetic screen, we discovered that the recognized effector from M. oryzae is Pathogenicity toward Weeping Lovegrass 2 (Pwl2), a host range determinant factor that prevents M. oryzae from infecting weeping lovegrass (Eragrostis curvula). Mla3 has therefore convergently evolved the capacity to recognize effectors from diverse pathogens. MLA3 confers dosage-dependent resistance to rice blast through recognition of the effector Pwl2, which contributes to host range dynamics. [ABSTRACT FROM AUTHOR]