소장자료
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001 | 0100799396▲ | ||
005 | 20240318103848▲ | ||
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008 | 240116s2023 us |||||||||||||||c||eng d▲ | ||
020 | ▼a9798379710651▲ | ||
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040 | ▼aMiAaPQ▼cMiAaPQ▲ | ||
082 | 0 | ▼a641▲ | |
100 | 1 | ▼aCarmody, Caitlin Marie.▼0(orcid)0000-0002-2295-1427▲ | |
245 | 1 | 0 | ▼aGenetically Engineered Bacteriophages for Biotechnology and Biomedicine Applications▼h[electronic resource]▲ |
260 | ▼a[S.l.]: ▼bCornell University. ▼c2023▲ | ||
260 | 1 | ▼aAnn Arbor : ▼bProQuest Dissertations & Theses, ▼c2023▲ | |
300 | ▼a1 online resource(277 p.)▲ | ||
500 | ▼aSource: Dissertations Abstracts International, Volume: 84-12, Section: B.▲ | ||
500 | ▼aAdvisor: Nugen, Sam.▲ | ||
502 | 1 | ▼aThesis (Ph.D.)--Cornell University, 2023.▲ | |
506 | ▼aThis item must not be sold to any third party vendors.▲ | ||
520 | ▼aBacteriophages' abundance and high specificity to infecting only bacteria hosts within their narrow host range have made them an ideal candidate for utilization in bacteria diagnostics and bacterial infection targeting therapeutics. Genetic and chemical modifications have been performed on bacteriophages to functionalize them with a variety of molecules including fluorophores, antigens, nanoparticles, drugs, and polymers to tailor their features for specific applications. In this work, the genetic engineering tool CRISPR-Cas9 was employed to facilitate modification of the large and complex genome of bacteriophage T4. This bacteriophage is well-characterized and infects Escherichia coli hosts making it an ideal candidate for modeling bacteriophage modifications. Throughout this work, T4 bacteriophages were genetically modified to functionalize them with luciferases for quantifiable signal production, affinity peptides for immobilization and conjugation, and chimeric tail fibers for altering host range. A diagnostic assay capable of detecting <10 CFU of E. coli in 100mLs of water in low-resource settings was developed utilizing one of the modified T4 bacteriophages as a biosensor. The genetic and phenotypic information gathered in this work can be applied to bacteriophages that infect bacteria strains prominent in agriculture and pharmaceutical industries to advance bacteriophage biotechnologies and biomedicines.▲ | ||
590 | ▼aSchool code: 0058.▲ | ||
650 | 4 | ▼aFood science.▲ | |
650 | 4 | ▼aGenetics.▲ | |
650 | 4 | ▼aBioengineering.▲ | |
653 | ▼aBacteriophage▲ | ||
653 | ▼aBiopharma▲ | ||
653 | ▼aBiotechnology▲ | ||
653 | ▼aGenetic engineering▲ | ||
653 | ▼aMicrobiology▲ | ||
690 | ▼a0359▲ | ||
690 | ▼a0369▲ | ||
690 | ▼a0202▲ | ||
710 | 2 | 0 | ▼aCornell University.▼bFood Science and Technology.▲ |
773 | 0 | ▼tDissertations Abstracts International▼g84-12B.▲ | |
773 | ▼tDissertation Abstract International▲ | ||
790 | ▼a0058▲ | ||
791 | ▼aPh.D.▲ | ||
792 | ▼a2023▲ | ||
793 | ▼aEnglish▲ | ||
856 | 4 | 0 | ▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16931570▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.▲ |
Genetically Engineered Bacteriophages for Biotechnology and Biomedicine Applications[electronic resource]
자료유형
국외eBook
서명/책임사항
Genetically Engineered Bacteriophages for Biotechnology and Biomedicine Applications [electronic resource]
발행사항
[S.l.] : Cornell University. 2023 Ann Arbor : ProQuest Dissertations & Theses , 2023
형태사항
1 online resource(277 p.)
일반주기
Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
Advisor: Nugen, Sam.
Advisor: Nugen, Sam.
학위논문주기
Thesis (Ph.D.)--Cornell University, 2023.
요약주기
Bacteriophages' abundance and high specificity to infecting only bacteria hosts within their narrow host range have made them an ideal candidate for utilization in bacteria diagnostics and bacterial infection targeting therapeutics. Genetic and chemical modifications have been performed on bacteriophages to functionalize them with a variety of molecules including fluorophores, antigens, nanoparticles, drugs, and polymers to tailor their features for specific applications. In this work, the genetic engineering tool CRISPR-Cas9 was employed to facilitate modification of the large and complex genome of bacteriophage T4. This bacteriophage is well-characterized and infects Escherichia coli hosts making it an ideal candidate for modeling bacteriophage modifications. Throughout this work, T4 bacteriophages were genetically modified to functionalize them with luciferases for quantifiable signal production, affinity peptides for immobilization and conjugation, and chimeric tail fibers for altering host range. A diagnostic assay capable of detecting <10 CFU of E. coli in 100mLs of water in low-resource settings was developed utilizing one of the modified T4 bacteriophages as a biosensor. The genetic and phenotypic information gathered in this work can be applied to bacteriophages that infect bacteria strains prominent in agriculture and pharmaceutical industries to advance bacteriophage biotechnologies and biomedicines.
주제
ISBN
9798379710651
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