소장자료
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082 | 0 | ▼a570▲ | |
100 | 1 | ▼aHope, Jen Marrero.▲ | |
245 | 1 | 0 | ▼aShining Light on TRKA: An Optobiological Approach to Dissecting Location-Specific TRKA Signaling▼h[electronic resource]▲ |
260 | ▼a[S.l.]: ▼bStanford University. ▼c2021▲ | ||
260 | 1 | ▼aAnn Arbor : ▼bProQuest Dissertations & Theses, ▼c2021▲ | |
300 | ▼a1 online resource(134 p.)▲ | ||
500 | ▼aSource: Dissertations Abstracts International, Volume: 85-03, Section: B.▲ | ||
500 | ▼aAdvisor: Cegelski, Lynette;Lin, Michael;Cui, Bianxiao.▲ | ||
502 | 1 | ▼aThesis (Ph.D.)--Stanford University, 2021.▲ | |
506 | ▼aThis item must not be sold to any third party vendors.▲ | ||
520 | ▼aNerve growth factor (NGF) and its primary receptor, tropomyosin receptor kinase A (TrkA), play a crucial role in neuronal development, differentiation, and survival. Aberrant TrkA signaling has been implicated in chronic neuropathic and nociceptive pain disorders, neurodegenerative disorders, and cancers. It has been hypothesized that after endocytosis of the active receptor, TrkA continues to signal from the endosome. Although neurotrophin biology has been an active field of study for almost 70 years, questions around the identity of the signaling endosome and the impact of the location and duration of receptor activation have remained unanswered, due to the limitations of traditional cell biology methods. In this work, we have leveraged the light-induced protein-protein interaction system iLID to construct opto-TrkA, a version of this receptor that is activated by blue light rather than its endogenous ligand. This system permits rapid, reversible, location-specific activation of TrkA in cellular systems as well as model organisms. We have demonstrated that opto-TrkA is capable of reproducing key hallmarks of endogenous TrkA signaling in a cellular model. We offer the system for use in dissecting the contributions of TrkA at different subcellular compartments to the overall cell response. Further, we have begun to demonstrate the utility of opto-TrkA in model organisms. It is our hope that this system will be an invaluable tool in expanding our understanding of neurotrophin biology.▲ | ||
590 | ▼aSchool code: 0212.▲ | ||
650 | 4 | ▼aGrowth factors.▲ | |
650 | 4 | ▼aNeurons.▲ | |
650 | 4 | ▼aClustering.▲ | |
650 | 4 | ▼aPhosphorylation.▲ | |
650 | 4 | ▼aCell culture.▲ | |
650 | 4 | ▼aCellular biology.▲ | |
690 | ▼a0379▲ | ||
710 | 2 | 0 | ▼aStanford University.▲ |
773 | 0 | ▼tDissertations Abstracts International▼g85-03B.▲ | |
773 | ▼tDissertation Abstract International▲ | ||
790 | ▼a0212▲ | ||
791 | ▼aPh.D.▲ | ||
792 | ▼a2021▲ | ||
793 | ▼aEnglish▲ | ||
856 | 4 | 0 | ▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16934423▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.▲ |
Shining Light on TRKA: An Optobiological Approach to Dissecting Location-Specific TRKA Signaling[electronic resource]
자료유형
국외eBook
서명/책임사항
Shining Light on TRKA: An Optobiological Approach to Dissecting Location-Specific TRKA Signaling [electronic resource]
발행사항
[S.l.] : Stanford University. 2021 Ann Arbor : ProQuest Dissertations & Theses , 2021
형태사항
1 online resource(134 p.)
일반주기
Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
Advisor: Cegelski, Lynette;Lin, Michael;Cui, Bianxiao.
Advisor: Cegelski, Lynette;Lin, Michael;Cui, Bianxiao.
학위논문주기
Thesis (Ph.D.)--Stanford University, 2021.
요약주기
Nerve growth factor (NGF) and its primary receptor, tropomyosin receptor kinase A (TrkA), play a crucial role in neuronal development, differentiation, and survival. Aberrant TrkA signaling has been implicated in chronic neuropathic and nociceptive pain disorders, neurodegenerative disorders, and cancers. It has been hypothesized that after endocytosis of the active receptor, TrkA continues to signal from the endosome. Although neurotrophin biology has been an active field of study for almost 70 years, questions around the identity of the signaling endosome and the impact of the location and duration of receptor activation have remained unanswered, due to the limitations of traditional cell biology methods. In this work, we have leveraged the light-induced protein-protein interaction system iLID to construct opto-TrkA, a version of this receptor that is activated by blue light rather than its endogenous ligand. This system permits rapid, reversible, location-specific activation of TrkA in cellular systems as well as model organisms. We have demonstrated that opto-TrkA is capable of reproducing key hallmarks of endogenous TrkA signaling in a cellular model. We offer the system for use in dissecting the contributions of TrkA at different subcellular compartments to the overall cell response. Further, we have begun to demonstrate the utility of opto-TrkA in model organisms. It is our hope that this system will be an invaluable tool in expanding our understanding of neurotrophin biology.
ISBN
9798380270816
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