소장자료
LDR | 02824cam a2200445 a 4500 | ||
001 | 0093720900▲ | ||
005 | 20180519175652▲ | ||
008 | 150528s2015 nyua b 001 0 eng c▲ | ||
010 | ▼a2015942639▲ | ||
020 | ▼a9781493927715 (hbk : alk. paper)▲ | ||
020 | ▼a149392771X (hbk : alk. paper)▲ | ||
020 | ▼a9781493927722 (eBook)▲ | ||
020 | ▼a1493927728 (eBook)▲ | ||
035 | ▼a(KERIS)REF000018177819▲ | ||
040 | ▼aYDXCP▼beng▼cNLM▼dYDXCP▼dIDL▼dNUI▼dIAL▼dOCLCO▼dOCLCQ▼dOCLCO▼dDLC▼d221016▲ | ||
042 | ▼anlmcopyc▼alccopycat▲ | ||
050 | 0 | 0 | ▼aQP517.C45▼bH44 2015▲ |
060 | 0 | 0 | ▼aW1 ME9616J▼bv.1322 2015▲ |
060 | 1 | 0 | ▼aQU 55.2▲ |
082 | 0 | 0 | ▼a571.7/4▼223▲ |
090 | ▼a571.74▼bH453r2▲ | ||
245 | 0 | 0 | ▼aHedgehog signaling protocols /▼cedited by Natalia A. Riobo.▲ |
250 | ▼a2nd ed.▲ | ||
260 | ▼aNew York :▼bHumana Press,▼c2015.▲ | ||
300 | ▼axvi, 200 p. :▼bill. ;▼c26 cm.▲ | ||
490 | 1 | ▼aMethods in molecular biology,▼x1064-3745 ;▼v1322▲ | |
490 | 1 | ▼aSpringer protocols▲ | |
504 | ▼aIncludes bibliographical references and index.▲ | ||
505 | 8 | 0 | ▼tLuciferase reporter assays to study transcriptional activity of hedgehog signaling in normal and cancer cells /▼rSilvia Pandolfi and Barbara Stecca --▼tMeasuring expression levels of endogenous gli genes by immunoblotting and real-time PCR /▼rPawel Niewiadomski and Rajat Rohatgi --▼tQuantitative immunoblotting of endogenous hedgehog pathway components /▼rShohreh F. Farzan and David J. Robbins --▼tMeasuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry /▼rRobert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi --▼tRapid screening of Gli2/3 mutants using the Flp-In system /▼rPawel Niewiadomski and Rajat Rohatgi --▼tInsights into gli factors ubiquitylation methods /▼rPaola Infante, Romina Alfonsi, and Lucia Di Marcotullio --▼tDetermination of acetylation of the gli transcription factors /▼rSonia Coni, Laura Di Magno, and Gianluca Canettieri.▲ |
505 | 8 | 0 | ▼tEfficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence /▼rJoão Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A. --▼tMethods for detection of Ptc1-driven LacZ expression in adult mouse skin /▼rDonna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo --▼tDetermination and analysis of cellular metabolic changes by noncanonical hedgehog signaling /▼rRaffaele Teperino and John Andrew Pospisilik.▲ |
650 | 0 | ▼aCellular signal transduction▼xResearch▼xMethodology.▲ | |
650 | 0 | ▼aTranscription factors▼xResearch▼xMethodology.▲ | |
650 | 1 | 2 | ▼aHedgehog Proteins.▲ |
650 | 2 | 2 | ▼aSignal Transduction.▲ |
650 | 2 | 2 | ▼aTranscription Factors.▲ |
655 | 2 | ▼aLaboratory Manuals.▲ | |
700 | 1 | ▼aRiobo, Natalia A.▲ | |
830 | 0 | ▼aMIMB ;▼v1322.▲ | |
830 | 0 | ▼aSpringer protocols (Series)▼x1949-2448▲ | |
999 | ▼a김진영▼c김미선▲ |
Hedgehog signaling protocols
자료유형
국외단행본
서명/책임사항
Hedgehog signaling protocols / edited by Natalia A. Riobo.
판사항
2nd ed.
발행사항
New York : Humana Press , 2015.
형태사항
xvi, 200 p. : ill. ; 26 cm.
총서사항
Methods in molecular biology , 1064-3745 ; 1322
Springer protocols
MIMB ; 1322.
Springer protocols (Series) 1949-2448
Springer protocols
MIMB ; 1322.
Springer protocols (Series) 1949-2448
서지주기
Includes bibliographical references and index.
내용주기
Luciferase reporter assays to study transcriptional activity of hedgehog signaling in normal and cancer cells / Silvia Pandolfi and Barbara Stecca -- Measuring expression levels of endogenous gli genes by immunoblotting and real-time PCR / Pawel Niewiadomski and Rajat Rohatgi -- Quantitative immunoblotting of endogenous hedgehog pathway components / Shohreh F. Farzan and David J. Robbins -- Measuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry / Robert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi -- Rapid screening of Gli2/3 mutants using the Flp-In system / Pawel Niewiadomski and Rajat Rohatgi -- Insights into gli factors ubiquitylation methods / Paola Infante, Romina Alfonsi, and Lucia Di Marcotullio -- Determination of acetylation of the gli transcription factors / Sonia Coni, Laura Di Magno, and Gianluca Canettieri.
Efficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence / João Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A. -- Methods for detection of Ptc1-driven LacZ expression in adult mouse skin / Donna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo -- Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / Raffaele Teperino and John Andrew Pospisilik.
Efficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence / João Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A. -- Methods for detection of Ptc1-driven LacZ expression in adult mouse skin / Donna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo -- Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / Raffaele Teperino and John Andrew Pospisilik.
주제
ISBN
9781493927715 (hbk : alk. paper) 149392771X (hbk : alk. paper) 9781493927722 (eBook) 1493927728 (eBook)
청구기호
571.74 H453r2
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