소장자료
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005 | 20240322165550▲ | ||
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020 | ▼a9798380395250▲ | ||
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040 | ▼aMiAaPQ▼cMiAaPQ▲ | ||
082 | 0 | ▼a616▲ | |
100 | 1 | ▼aPayne, Anja.▲ | |
245 | 1 | 0 | ▼aActivity-Dependent Reorganization of Inhibition by the Inducible Transcription Factor, NPAS4, and the Effects on CA1 Place Cell Activity▼h[electronic resource]▲ |
260 | ▼a[S.l.]: ▼bUniversity of California, San Diego. ▼c2023▲ | ||
260 | 1 | ▼aAnn Arbor : ▼bProQuest Dissertations & Theses, ▼c2023▲ | |
300 | ▼a1 online resource(107 p.)▲ | ||
500 | ▼aSource: Dissertations Abstracts International, Volume: 85-03, Section: B.▲ | ||
500 | ▼aAdvisor: Bloodgood, Brenda.▲ | ||
502 | 1 | ▼aThesis (Ph.D.)--University of California, San Diego, 2023.▲ | |
506 | ▼aThis item must not be sold to any third party vendors.▲ | ||
520 | ▼aIn order to form a memory, transient experiences are captured by neurons in the brain and transformed into long-lasting changes in cell circuitry and connectivity. To elucidate the mechanisms underlying memory formation it is necessary to determine how pyramidal neuron (PN) activity is transformed into changes in the future output of the neuron. NPAS4, an immediate early gene that is expressed transiently following PN activity, has been linked to changes in inhibitory synaptic connectivity. Specifically, NPAS4 leads to recruitment of somatic CCK (cholecystokinin+) basket cell synapses and destabilization of CCK dendritic inhibitory synapses. This cell-autonomous regulation of inhibitory synapses indicates that NPAS4 plays a role in shaping CA1 PN activity in vivo but, to date, there are no studies investigating this. Here we use an optotagging approach to compare the in vivo activity of simultaneously recorded, intermingled, NPAS4 wild type and knockout (KO) CA1 PNs from freely moving mice. We find that NPAS4 KO neurons have impaired spatial tuning and that this is accompanied by deficits in the stability of their firing across the session. Furthermore, NPAS4 KO neurons are less bursty within the place field. This reduction in bursting has implications for other aspects of spike timing including theta-coupling and phase precession. Specifically, we find that NPAS4 KO neurons are less theta-coupled within the place field and that their phase precession slopes are more shallow. Taken together our results demonstrate that NPAS4, through the reorganization of inhibitory synapses, is important for both the tuning of place fields in CA1 and for the refinement of sequences.▲ | ||
590 | ▼aSchool code: 0033.▲ | ||
650 | 4 | ▼aNeurosciences.▲ | |
650 | 4 | ▼aCellular biology.▲ | |
653 | ▼aHippocampus▲ | ||
653 | ▼aInhibition▲ | ||
653 | ▼aCell activity▲ | ||
653 | ▼aPyramidal neuron▲ | ||
653 | ▼aCell-autonomous regulation▲ | ||
690 | ▼a0317▲ | ||
690 | ▼a0379▲ | ||
710 | 2 | 0 | ▼aUniversity of California, San Diego.▼bNeurosciences.▲ |
773 | 0 | ▼tDissertations Abstracts International▼g85-03B.▲ | |
773 | ▼tDissertation Abstract International▲ | ||
790 | ▼a0033▲ | ||
791 | ▼aPh.D.▲ | ||
792 | ▼a2023▲ | ||
793 | ▼aEnglish▲ | ||
856 | 4 | 0 | ▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16933844▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.▲ |
Activity-Dependent Reorganization of Inhibition by the Inducible Transcription Factor, NPAS4, and the Effects on CA1 Place Cell Activity[electronic resource]
자료유형
국외eBook
서명/책임사항
Activity-Dependent Reorganization of Inhibition by the Inducible Transcription Factor, NPAS4, and the Effects on CA1 Place Cell Activity [electronic resource]
개인저자
발행사항
[S.l.] : University of California, San Diego. 2023 Ann Arbor : ProQuest Dissertations & Theses , 2023
형태사항
1 online resource(107 p.)
일반주기
Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
Advisor: Bloodgood, Brenda.
Advisor: Bloodgood, Brenda.
학위논문주기
Thesis (Ph.D.)--University of California, San Diego, 2023.
요약주기
In order to form a memory, transient experiences are captured by neurons in the brain and transformed into long-lasting changes in cell circuitry and connectivity. To elucidate the mechanisms underlying memory formation it is necessary to determine how pyramidal neuron (PN) activity is transformed into changes in the future output of the neuron. NPAS4, an immediate early gene that is expressed transiently following PN activity, has been linked to changes in inhibitory synaptic connectivity. Specifically, NPAS4 leads to recruitment of somatic CCK (cholecystokinin+) basket cell synapses and destabilization of CCK dendritic inhibitory synapses. This cell-autonomous regulation of inhibitory synapses indicates that NPAS4 plays a role in shaping CA1 PN activity in vivo but, to date, there are no studies investigating this. Here we use an optotagging approach to compare the in vivo activity of simultaneously recorded, intermingled, NPAS4 wild type and knockout (KO) CA1 PNs from freely moving mice. We find that NPAS4 KO neurons have impaired spatial tuning and that this is accompanied by deficits in the stability of their firing across the session. Furthermore, NPAS4 KO neurons are less bursty within the place field. This reduction in bursting has implications for other aspects of spike timing including theta-coupling and phase precession. Specifically, we find that NPAS4 KO neurons are less theta-coupled within the place field and that their phase precession slopes are more shallow. Taken together our results demonstrate that NPAS4, through the reorganization of inhibitory synapses, is important for both the tuning of place fields in CA1 and for the refinement of sequences.
주제
ISBN
9798380395250
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