학술논문

Bovine placentome preservation for light microscopy evaluation
Document Type
article
Source
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. April 2000 52(2)
Subject
Bovine
placentome
light microscopy
Language
English
ISSN
0102-0935
Abstract
This work evaluates the preservation of bovine placentomes using paraformaldehyde and glutaraldehyde at 4% and solutions of Karnovsky, Bouin and Carnoy for 4, 12 and 24 hours fixation, at room temperature or at 4ºC, before inclusion in plastic resin and paraffin. The best preservation of the specimens was obtained after 12 hours of fixation with those solutions containing aldehydes. With Bouin solution the best time of fixation was 4 hours, while for Carnoy solution the best time of fixation was 24 hours. Inclusion in plastic resin gave better results than in paraffin, and the temperature did not influence the quality of preservation of the specimens of bovine placentomes for evaluation with light microscopy.